M Bruno Sargueil » Equipe : Mécanismes moléculaires de la traduction de l’ARN viral

Activité de recherche de l’équipe : Mécanismes moléculaires de la traduction de l’ARN viral

M Bruno Sargueil

Directeur de Recherche – Responsable de l’équipe CNRSCiTCoM
Tel :: Numéro

Informations

Recherche  :

  • Structure des ARN
  • Traduction des ARNm viraux
  • Interaction ARN protéines
  • Conduite de projets pluridisciplinaires

Approches utilisées :

  • Détermination des structures d’ARN (Sondes chimiques et enzymatiques/SHAPE/Approche systématique automatisée)
  • Reconstitution in vitro / Biochimie
  • SELEX (evolution Darwinienne in vitro)
  • Chimie génétique
  • Automatisation des process

29 documents

Articles dans une revue21 documents

  • Camélia Kitoun, Saidbakhrom Saidjalolov, Delphine Bouquet, Fabiola Djago, Quentin Blancart Remaury, et al.. Traceless Staudinger Ligation to Access Stable Aminoacyl- or Peptidyl-Dinucleotide. ACS Omega, 2023, 8 (4), pp.3850-3860. ⟨10.1021/acsomega.2c06135⟩. ⟨hal-04294940⟩
  • Jenniffer Angulo, C. Joaquín Cáceres, Nataly Contreras, Leandro Fernández-García, Nathalie Chamond, et al.. Polypyrimidine-Tract-Binding Protein Isoforms Differentially Regulate the Hepatitis C Virus Internal Ribosome Entry Site. Viruses, 2023, 15 (1), pp.8. ⟨10.3390/v15010008⟩. ⟨hal-04275415⟩
  • Rodrigo Coronel-Tellez, Mateusz Pospiech, Maxime Barrault, Wenfeng Liu, Valérie Bordeau, et al.. sRNA-controlled iron sparing response in Staphylococci. Nucleic Acids Research, 2022, 50 (15), pp.8529-8546. ⟨10.1093/nar/gkac648⟩. ⟨hal-03775296v2⟩
  • Afaf Saaidi, Delphine M Allouche, Mireille Regnier, Bruno Sargueil, Yann Ponty. IPANEMAP: Integrative Probing Analysis of Nucleic Acids Empowered by Multiple Accessibility Profiles. Nucleic Acids Research, 2020, 48 (15), pp.8276--8289. ⟨10.1093/nar/gkaa607⟩. ⟨hal-02889041v3⟩
  • Elisa Frezza, Antoine Courban, Delphine M Allouche, Bruno Sargueil, Samuela Pasquali. The interplay between molecular flexibility and RNA chemical probing reactivities analyzed at the nucleotide level via an extensive molecular dynamics study. Methods, 2019, 162-163, pp.108-127. ⟨10.1016/j.ymeth.2019.05.021⟩. ⟨hal-02387476⟩
  • C joaquín Cáceres, Jenniffer Angulo, Fernando Lowy, Nataly Contreras, Beth Walters, et al.. Non-canonical translation initiation of the spliced mRNA encoding the human T-cell leukemia virus type 1 basic leucine zipper protein. Nucleic Acids Research, 2019, 47 (3), pp.1599-1599. ⟨10.1093/nar/gky1249⟩. ⟨hal-02387485⟩
  • Grégoire de Bisschop, Melissa Ameur, Nathalie Ulryck, Fatima Benattia, Luc Ponchon, et al.. HIV-1 gRNA, a biological substrate, uncovers the potency of DDX3X biochemical activity. Biochimie, 2019, 164, pp.83-94. ⟨10.1016/j.biochi.2019.03.008⟩. ⟨hal-02325540⟩
  • Valérie Pezo, Camille Hassan, Dominique Louis, Bruno Sargueil, Piet Herdewijn, et al.. Metabolic Recruitment and Directed Evolution of Nucleoside Triphosphate Uptake in Escherichia coli. ACS Synthetic Biology, 2018, 7 (6), pp.1565-1572. ⟨10.1021/acssynbio.8b00048⟩. ⟨hal-02387495⟩
  • Margaret Willcocks, Salmah Zaini, Nathalie Chamond, Nathalie Ulryck, Delphine M Allouche, et al.. Distinct roles for the IIId2 sub-domain in pestivirus and picornavirus internal ribosome entry sites. Nucleic Acids Research, 2017, 45 (22), pp.13016-13028. ⟨10.1093/nar/gkx991⟩. ⟨hal-02523078⟩
  • Jules Deforges, Sylvain de Breyne, Melissa Ameur, Nathalie Ulryck, Nathalie Chamond, et al.. Two ribosome recruitment sites direct multiple translation events within HIV1 Gag open reading frame. Nucleic Acids Research, 2017, 45 (12), pp.7382--7400. ⟨10.1093/nar/gkx303⟩. ⟨hal-01505282⟩
  • Jenniffer Angulo, Nathalie Ulryck, Jules Deforges, Nathalie Chamond, Marcelo López-Lastra, et al.. LOOP IIId of the HCV IRES is essential for the structural rearrangement of the 40S-HCV IRES complex. Nucleic Acids Research, 2016, 44 (3), pp.1309-1325. ⟨10.1093/nar/gkv1325⟩. ⟨hal-02523090⟩
  • Nathalie Chamond, Jules Deforges, Nathalie Ulryck, Bruno Sargueil. 40S recruitment in the absence of eIF4G/4A by EMCV IRES refines the model for translation initiation on the archetype of Type II IRESs.. Nucleic Acids Research, 2014, 42 (16), pp.10373-84. ⟨10.1093/nar/gku720⟩. ⟨hal-01067767⟩
  • Clémentine Delan-Forino, Jules Deforges, Lionel Benard, Bruno Sargueil, Marie-Christine Maurel, et al.. Structural Analyses of Avocado sunblotch viroid Reveal Differences in the Folding of Plus and Minus RNA Strands. Viruses, 2014, 6 (2), pp.489-506. ⟨10.3390/v6020489⟩. ⟨hal-01343316⟩
  • Nathalie Chamond, Jules Deforges, Nathalie Ulryck, Bruno Sargueil. 40S recruitment in the absence of eIF4G/4A by EMCV IRES refines the model for translation initiation on the archetype of Type II IRESs. Nucleic Acids Research, 2014, 42, pp.10373 - 10384. ⟨10.1093/nar/gku720⟩. ⟨hal-01075147⟩
  • Amira Souii, Manel M'Hadheb-Gharbi, Bruno Sargueil, Audrey Brossard, Nathalie Chamond, et al.. Ribosomal Initiation Complex Assembly within the Wild-Strain of Coxsackievirus B3 and Live-Attenuated Sabin3-like IRESes during the Initiation of Translation. International Journal of Molecular Sciences, 2013, 14 (3), pp.4400-4418. ⟨10.3390/ijms14034400⟩. ⟨hal-02523097⟩
  • Sylvain de Breyne, Nathalie Chamond, Didier Décimo, Mary-Anne Trabaud, Patrice André, et al.. In vitro studies reveal that different modes of initiation on HIV-1 mRNA have different levels of requirement for eukaryotic initiation factor 4F.. FEBS Journal, 2012, 279 (17), pp.3098-111. ⟨10.1111/j.1742-4658.2012.08689.x⟩. ⟨hal-00965629⟩
  • Jules Deforges, Nathalie Chamond, Bruno Sargueil. Structural investigation of HIV-1 genomic RNA dimerization process reveals a role for the Major Splice-site Donor stem loop. Biochimie, 2012, 94 (7), pp.1481-1489. ⟨10.1016/j.biochi.2012.02.009⟩. ⟨hal-02544383⟩
  • Nicolas Locker, Nathalie Chamond, Bruno Sargueil. A conserved structure within the HIV gag open reading frame that controls translation initiation directly recruits the 40S subunit and eIF3. Nucleic Acids Research, 2011, 39 (6), pp.2367-2377. ⟨10.1093/nar/gkq1118⟩. ⟨hal-02544362⟩
  • Nathalie Chamond, Nicolas Locker, Bruno Sargueil. The different pathways of HIV genomic RNA translation. Biochemical Society Transactions, 2010, 38 (6), pp.1548-1552. ⟨10.1042/BST0381548⟩. ⟨hal-02544365⟩
  • Laure Weill, Laurie James, Nathalie Ulryck, Nathalie Chamond, Cecile Herbreteau, et al.. A new type of IRES within gag coding region recruits three initiation complexes on HIV-2 genomic RNA. Nucleic Acids Research, 2010, 38 (4), pp.1367-1381. ⟨10.1093/nar/gkp1109⟩. ⟨hal-02544350⟩
  • Laurent Balvay, Marcelo Lopez Lastra, Bruno Sargueil, Jean-Luc Darlix, Théophile Ohlmann. Translational control of retroviruses.. Nat Rev Microbiol, 2007, 5 (2), pp.128-40. ⟨10.1038/nrmicro1599⟩. ⟨hal-00136396⟩

Communications dans un congrès3 documents

  • Afaf Saaidi, Yann Ponty, Mathieu Blanchette, Mireille Regnier, Bruno Sargueil. An EM algorithm for mapping short reads in multiple RNA structure probing experiments. Matbio2017, King's College London Sep 2017, London, United Kingdom. ⟨hal-01590528⟩
  • Afaf Saaidi, Yann Ponty, Bruno Sargueil. An integrative approach for predicting the RNA secondary structure for the HIV–1 Gag UTR using probing data. JOBIM - Journées Ouvertes en Biologie, Informatique et Mathématiques - 2017, Jul 2017, Lille, France. pp.102. ⟨hal-01534587⟩
  • Afaf Saaidi, Delphine Allouche, Bruno Sargueil, Yann Ponty. Towards structural models for the Ebola UTR regions using experimental SHAPE probing data. JOBIM - Journées Ouvertes en Biologie, Informatique et Mathématiques - 2016, Jun 2016, Lyon, France. ⟨hal-01332469⟩

Poster de conférence1 document

  • Afaf Saaidi, Delphine M Allouche, Yann Ponty, Bruno M Sargueil, Mireille M Regnier. Computational methods for comparing and integrating multiple probing assays to predict RNA secondary structure . Doctorial journey Interface, Ecole polytechnique, Palaiseau, Nov 2016, Palaiseau, France. 10. ⟨hal-01558227⟩

Chapitres d'ouvrage2 documents

  • Bruno Sargueil, Grégoire de Bisschop. RNA Footprinting Using Small Chemical Reagents. Luc Ponchon. RNA Scaffolds: Methods and Protocols, 2323, Springer US, pp.13-23, 2021, Methods in Molecular Biology, 978-1-0716-1498-3. ⟨10.1007/978-1-0716-1499-0_2⟩. ⟨hal-03450546⟩
  • Delphine M Allouche, Grégoire de Bisschop, Afaf Saaidi, Yann Ponty, Sargueil Bruno. RNA secondary structure modelling following the IPANEMAP workflow. RNA Folding - Methods and Protocols, Springer Nature, In press, Methods in Molecular Biology. ⟨hal-02324783⟩

Pré-publications, Documents de travail2 documents

  • Jorge Fernandez-De-Cossio-Diaz, Pierre Hardouin, Francois-Xavier Lyonnet Du Moutier, Andrea Di Gioacchino, Bertrand Marchand, et al.. Designing molecular RNA switches with Restricted Boltzmann machines. 2023. ⟨hal-04294884⟩
  • Camila Pereira-Montecinos, Daniela Toro-Ascuy, Cecilia Rojas-Fuentes, Sebastián Riquelme-Barrios, Bárbara Rojas-Araya, et al.. An epitranscriptomic switch at the 5´-UTR controls genome selection during HIV-1 genomic RNA packaging. 2020. ⟨hal-03020001⟩

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