M Jean-François Gaucher » Equipe : Signalisation et transport membranaire

Informations

Recherche :

• Disciplines :
  • Biologie Structurale & Biophysique
•  Outils :
  • Biologie Structurale : cristallographie des macromolécules, SAXS, dichroïsme circulaire, modélisation moléculaire
  • Biochimie : biologie moléculaire, expression procaryotes des protéines, renaturation de protéines insolubles, solubilisation de protéines membranaires (détergents- SMALPS), techniques biochimiques de purification et d’analyse.
  • Biophysique : ITC, électrophysiologie, fluorescence

• Thèmes de recherche :
  • Développement de molécules peptidiques ciblant les VEGF et leurs récepteurs, à visée thérapeutique ou d’analyse.
  • Développement d’outils biochimiques d’analyse du fonctionnement de la pompe tripartite MacAB-TolC

• Collaborations :

– Dr. Sylvain Broussy, CitCom (CNRS – UMR 8038), Université de Paris

– Pr. Ulrich Zachariae, Computational Biology, College of Life sciences, University of Dundee (UK)

– Dr. Samantha Pitt, School of Medecine, University of St . Andrews (UK)


Publications :

• VEGF (Vascular Endothelial Growth Factor) Functionalized Magnetic Beads in a Microfluidic Device to Improve the Angiogenic Balance in Preeclampsia. Trapiella-Alfonso L, Alexandre L, Fraichard C, Pons K, Dumas S, Huart L, Gaucher JF, Hebert-Schuster M, Guibourdenche J, Fournier T, Vidal M, Broutin I, Lecomte-Raclet L, Malaquin L, Descroix S, Tsatsaris V, Gagey-Eilstein N, Lecarpentier E. Hypertension. 2019 Jul;74(1):145-153. doi: 10.1161/HYPERTENSIONAHA.118.12380. Epub 2019 May 13. PMID: 31079531
• VEGFR1 domain 2 covalent labeling with horseradish peroxidase: Development of a displacement assay on VEGF. Reille-Seroussi M, Gaucher JF, Cussac LA, Broutin I, Vidal M, Broussy S. Anal Biochem. 2017 Aug 1;530:107-112. doi: 10.1016/j.ab.2017.05.004. Epub 2017 May 3. PMID: 28477965
• Biophysical Studies of the Induced Dimerization of Human VEGF Receptor 1 Binding Domain by Divalent Metals Competing with VEGF-A. Gaucher JF, Reille-Seroussi M, Gagey-Eilstein N, Broussy S, Coric P, Seijo B, Lascombe MB, Gautier B, Liu WQ, Huguenot F, Inguimbert N, Bouaziz S, Vidal M, Broutin I. PLoS One. 2016 Dec 12;11(12):e0167755. doi: 10.1371/journal.pone.0167755. eCollection 2016. PMID: 27942001
• Vascular Endothelial Growth Factor Peptide Ligands Explored by Competition Assay and Isothermal Titration Calorimetry. Reille-Seroussi M, Gaucher JF, Desole C, Gagey-Eilstein N, Brachet F, Broutin I, Vidal M, Broussy S. Biochemistry. 2015 Aug 25;54(33):5147-56. doi: 10.1021/acs.biochem.5b00722. Epub 2015 Aug 13. PMID: 26222917
• Targeting the proangiogenic VEGF-VEGFR protein-protein interface with drug-like compounds by in silico and in vitro screening. Gautier B, Miteva MA, Goncalves V, Huguenot F, Coric P, Bouaziz S, Seijo B, Gaucher JF, Broutin I, Garbay C, Lesnard A, Rault S, Inguimbert N, Villoutreix BO, Vidal M. Chem Biol. 2011 Dec 23;18(12):1631-9. doi: 10.1016/j.chembiol.2011.10.016. PMID: 22195565
• Interactions of isolated C-terminal fragments of neural Wiskott-Aldrich syndrome protein (N-WASP) with actin and Arp2/3 complex. Gaucher JF, Maugé C, Didry D, Guichard B, Renault L, Carlier MF. J Biol Chem. 2012 Oct 5;287(41):34646-59. doi: 10.1074/jbc.M112.394361. Epub 2012 Jul 30. PMID: 2284700
• Interactions of a new alpha-aminophosphinic derivative inside the active site of TLN (thermolysin): a model for zinc-metalloendopeptidase inhibition. Selkti M, Tomas A, Gaucher JF, Prangé T, Fournié-Zaluski MC, Chen H, Roques BP. Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1200-5. doi: 10.1107/s0907444903010060. Epub 2003 Jun 27. PMID: 12832763
• Crystal structure of apo-calmodulin bound to the first two IQ motifs of myosin V reveals essential recognition features. Houdusse A, Gaucher JF, Krementsova E, Mui S, Trybus KM, Cohen C. Version 2. Proc Natl Acad Sci U S A. 2006 Dec 19;103(51):19326-31. doi: 10.1073/pnas.0609436103. Epub 2006 Dec 6. PMID: 17151196
• Arp2/3 controls the motile behavior of N-WASP-functionalized GUVs and modulates N-WASP surface distribution by mediating transient links with actin filaments. Delatour V, Helfer E, Didry D, Lê KH, Gaucher JF, Carlier MF, Romet-Lemonne G. Biophys J. 2008 Jun;94(12):4890-905. doi: 10.1529/biophysj.107.118653. Epub 2008 Mar 7. PMID: 18326652
• The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate. Gaucher JF, Selkti M, Prangé T, Tomas A. Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2198-200. doi: 10.1107/s0907444902015457. Epub 2002 Nov 23. PMID: 12454500
• High resolution x-ray analysis of two mutants of a curaremimetic snake toxin. Gaucher JF, Ménez R, Arnoux B, Pusset J, Ducruix A. Eur J Biochem. 2000 Mar;267(5):1323-9. doi: 10.1046/j.1432-1327.2000.01099.x. PMID: 10691969
• Structures of escherichia coli CMP kinase alone and in complex with CDP: a new fold of the nucleoside monophosphate binding domain and insights into cytosine nucleotide specificity. Briozzo P, Golinelli-Pimpaneau B, Gilles AM, Gaucher JF, Burlacu-Miron S, Sakamoto H, Janin J, Bârzu O. Structure. 1998 Dec 15;6(12):1517-27. doi: 10.1016/s0969-2126(98)00150-6. PMID: 9862805
• Solubility diagram of the Rhodobacter sphaeroides reaction center as a function of PEG concentration. Gaucher JF, Riès-Kautt M, Reiss-Husson F, Ducruix A. FEBS Lett. 1997 Jan 20;401(2-3):113-6. doi: 10.1016/s0014-5793(96)01446-9. PMID: 9013869
• Crystal structures of alpha-mercaptoacyldipeptides in the thermolysin active site: structural parameters for a Zn monodentation or bidentation in metalloendopeptidases. Gaucher JF, Selkti M, Tiraboschi G, Prangé T, Roques BP, Tomas A, Fournié-Zaluski MC. Biochemistry. 1999 Sep 28;38(39):12569-76. doi: 10.1021/bi991043z. PMID: 10504225
• Crystal structure of bis( -chloro)bis[chloro-(o-phenanthroline-iV ‘)- СОррег( )], CU2(Cl2H8N2)2(Cl2)2 Viossat B, Gaucher JF, Mazurier A, Selkti M and Tomas A Zeitschrift für Kristallographie, 1998 Apr 01 ; 213: 329-330 doi:10.1524/ncrs.1998.213.14.343
• Structure of the photochemical reaction centre of a spheroidene-containing purple-bacterium, Rhodobacter sphaeroides Y, at 3 A resolution. Arnoux B, Gaucher JF, Ducruix A, Reiss-Husson F. Acta Crystallogr D Biol Crystallogr. 1995 May 1;51(Pt 3):368-79. doi: 10.1107/S0907444994013120. PMID: 15299304