M Luc Ponchon
Associate Professor University Paris CitéCiTCoMInformations
Research Interests
- – RNA-ligands interactions
- – Structural studies of RNA and proteins
- – In vivo RNA production
Selected Publications
(13) – Ranaei-Siadat E, Mérigoux C, Seijo B, Ponchon L, Saliou JM, Bernauer J, Sanglier-Cianférani S, Dardel F, Vachette P, Nonin-Lecomte S. In vivo tmRNA protection by SmpB and pre-ribosome binding conformation in solution. RNA. 2014 Oct;20(10):1607-20
(12) – Zivanovic Y, Confalonieri F, Ponchon L, Lurz R, Chami M, Flayhan A, Renouard M, Huet A, Decottignies P, Davidson AR, Breyton C, Boulanger P. Insights into bacteriophage T5 structure from the analysis of its morphogenesis genes and protein components. J Virol. 2014 Jan;88(2):1162-74
(11) – Ponchon L, Catala M, Seijo B, El Khouri M, Dardel F, Nonin-Lecomte S, Tisné C. Co-expression of RNA-protein complexes in Escherichia coli and applications to RNA biology. Nucleic Acids Res. 2013 Aug;41(15):e150.
(10) – Ponchon L, Beauvais G, Nonin-Lecomte S, Dardel F. Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold. Methods Mol Biol. 2012;941:9-18.
(9) – Ponchon L, Dardel F. Purification of RNA Expressed In Vivo Inserted in a tRNA Scaffold. Methods Mol Biol. 2012;941:1-8.
(8) – Ponchon L, Dardel F. Large scale expression and purification of recombinant RNA in Escherichia coli. Methods. 2011 Jun;54(2):267-73.
(7) – Ponchon L, Beauvais G, Nonin-Lecomte S, Dardel F. A generic protocol for the expression and purification of recombinant RNA in Escherichia coli using a tRNA scaffold. Nat Protoc. 2009;4(6):947-59.
(6) – Nonin-Lecomte*, S., Germaint -Amiot, N., Gillet, R., Hallier, M., Ponchon, L., Dardel, F. and Felden, B.Ribosome hijacking : a role for small protein B during trans-translation.EMBO reports 2009 10, 2, 160-165
(5) – Ponchon L, Dardel F.Recombinant RNA technology : the tRNA scaffold.Nat Methods. 2007 Jul ;4(7):571-6.
(4) – Ponchon L, Boulanger P, Labesse G, Letellier L. The endonuclease domain of bacteriophage terminases belongs to the resolvase/integrase/ribonuclease H superfamily : a bioinformatics analysis validated by a functional study on bacteriophage T5. J Biol Chem. 2006 Mar 3 ;281(9):5829-36. Epub 2005 Dec 22.
(3) – Ponchon L, Mangenot S, Boulanger P, Letellier L. Encapsidation and transfer of phage DNA into host cells : from in vivo to single particles studies. Biochim Biophys Acta. 2005 Aug 5 ;1724(3):255-61. Review.
(2) – Ponchon L, Dumas C, Kajava AV, Fesquet D, Padilla A.NMR solution structure of Mob1, a mitotic exit network protein and its interaction with an NDR kinase peptide. J Mol Biol. 2004 Mar 12 ;337(1):167-82.
(1) – Ponchon L, Dumas C, Fesquet D, Padilla A. Resonance assignments and topology of the 15N, 13C labelled 23 kDa core domain of xenopus Mob1. J Biomol NMR. 2004 Mar ;28(3):299-300.