Biographical Info
Research :
Techniques:
- Cloning, expression and purification of recombinant proteins
- Crystallogenesis
- X-ray crystallography, Cryo-EM
Study topics:
- Pseudopaline (metallophore) transport pathway in Pseudomonas aeruginosa
- Membrane proteins CntI (DMT) and MexB (RND), efflux pump MexAB-OprM
- Bacterial antibiotic resistance
Selected publications :
- Lhospice, Sébastien, Nicolas Oswaldo Gomez, Laurent Ouerdane, Catherine Brutesco, Ghassan Ghssein, Christine Hajjar, Ahmed Liratni, et al. 2017. « Pseudomonas Aeruginosa Zinc Uptake in Chelating Environment Is Primarily Mediated by the Metallophore Pseudopaline ». Scientific Reports 7 (1): 17132. https://doi.org/10.1038/s41598-017-16765-9.
- Gomez, Nicolas Oswaldo, Alexandre Tetard, Laurent Ouerdane, Clémentine Laffont, Catherine Brutesco, Geneviève Ball, Ryszard Lobinski, et al. 2021. « Involvement of the Pseudomonas Aeruginosa MexAB–OprM Efflux Pump in the Secretion of the Metallophore Pseudopaline ». Molecular Microbiology 115 (1): 84‑98. https://doi.org/10.1111/mmi.14600.
- Tsuchiya, Hirotoshi, Shintaro Doki, Mizuki Takemoto, Tatsuya Ikuta, Takashi Higuchi, Keita Fukui, Yoshihiro Usuda, et al. 2016. « Structural Basis for Amino Acid Export by DMT Superfamily Transporter YddG ». Nature 534 (7607): 417‑20. https://doi.org/10.1038/nature17991.
- Laffont, Clémentine, et Pascal Arnoux. 2020. « The Ancient Roots of Nicotianamine: Diversity, Role, Regulation and Evolution of Nicotianamine-like Metallophores ». Metallomics 12 (10): 1480‑93. https://doi.org/10.1039/D0MT00150C.
